The U.S. President’s Emergency Plan for AIDS Relief (PEPFAR), together with the Joint United Nations Programme on HIV/AIDS (UNAIDS), launched an initiative in 2015 to strengthen collaboration with faith-based partners in response to The Lancet Series on faith-based health care and the UNAIDS The Gap Report. The purpose of the FBO initiative was scaling up partnerships with faith-based organizations (FBOs) and addressing issues highlighted as important by The Lancet Series and the The Gap Report. 1,2,3 This report describes the work of the PEPFAR/ UNAIDS FBO Initiative between 2015 and 2019.